Impact of pymetrozine on glassy-winged sharpshooter feeding behavior and rate of Xylella fastidiosa transmission

Impact of pymetrozine on glassy-winged sharpshooter feeding behavior and rate of Xylella fastidiosa transmission

Pymetrozine is a compound that interferes with insect feeding and interrupts transmission of plant pathogens. The glassy-winged sharpshooter, Homalodisca coagulata Say (Hemiptera, Cicadellidae), is a vector of Xylella fastidiosa, the foregut-borne, propagative bacterium that causes Pierce's disease of grapevine. In this study, we recorded the behavioral response of H. coagulata to plants treate...

Glassy-winged Sharpshooter Transmission of Xylella fastidiosa to Plants

IMPACT OF MULTIPLE-STRAIN INFECTIONS OF XYLELLA FASTIDIOSA ON ACQUISITION AND TRANSMISSION BY THE GLASSY-WINGED SHARPSHOOTER Project Leaders:

In this project, studies were conducted to 1) develop a method to detect and differentiate multiple strains of Xylella fastidiosa in individual glassy-winged sharpshooters (GWSS,) and 2) determine the relative ability of an individual GWSS to simultaneously retain or transmit two strains of X. fastidiosa. Strain-specific primers were developed that can detect and differentiate the Pierce’s dise...

Detection of the Bacterium, Xylella fastidiosa, in Saliva of Glassy-Winged Sharpshooter, Homalodisca vitripennis

Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), the glassy-winged sharpshooter, is one of the most important vectors of the bacterium, Xylella fastidiosa subsp. piercei (Xanthomonadales: Xanthomonadaceae) that causes Pierce's Disease in grapevines in California. In the present study we report a new method for studying pathogen transmission or probing behavior of H. vitripennis. When...

DEVELOPING A METHOD TO DETECT XYLELLA FASTIDIOSA IN THE GLASSY-WINGED SHARPSHOOTER Project Leaders:

Dependable detection of Xylella fastidiosa (Xf) in glassy-winged sharpshooters (GWSS) is imperative for understanding Xf epidemiology and optimizing grapevine protection strategies. In this study, we have developed methods for extracting Xf DNA from GWSS vectors and optimized a SYBR green I based real-time PCR detection protocol that is fast, consistent, and inexpensive. The Qiagen DNeasy Tissu...